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Image Search Results
Journal: PLOS ONE
Article Title: Development and characterization of nanobodies that specifically target the oncogenic Phosphatase of Regenerating Liver-3 (PRL-3) and impact its interaction with a known binding partner, CNNM3
doi: 10.1371/journal.pone.0285964
Figure Lengend Snippet: (A) Nanobody 19 pulls down 3XFLAG-PRL-3 with minimal to no pulldown of 3XFLAG-PRL-1 or 3XFLAG-PRL-2. Successful nanobody coupling to Dynabeads was verified using an antibody against the C-terminal 6XHis-tag present on each nanobody. (B) Nanobody 19 pulls down HA-PRL-3 from HCT116 cells just as well as commercially available antibodies MAB3219 and sc-130355, and better than ab50276 and GTX100600. The input, transiently overexpressed HA-PRL-3, is shown separately. Nanobody 19 was coupled to superparamagnetic Dynabeads® M-270 Epoxy beads and used in immunoprecipitation assays with lysates from HEK293T cells transduced with 3XFLAG-PRL-1, -2 or -3 and HCT116 cells transiently transfected with HA-PRL-3. All anti-PRL-3 antibodies were incubated with HA-PRL-3 lysate and pulled down using Protein A and Protein G beads.
Article Snippet: We next examined the ability of nanobody 19 to immunoprecipitate HA-PRL-3 in comparison to four commercially available anti-PRL-3 antibodies: ab50276 (Abcam), GTX100600 (GeneTex),
Techniques: Immunoprecipitation, Transduction, Transfection, Incubation